Active Recombinant Human MAOB Protein

• Cat.No.: MAOB-109H
• Product Overview: Recombinant human MAOB protein without tag was expressed in insect cells.

Specification

• Description: The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine.
• Source: Insect cells
• Species: Human
• Bio-activity: Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 90 mM potassium phosphate (pH 7.4))
  Substrate: Kynuramine (4-hydroxyquinoline formation)
  Linear with time up to: 30 min(Determined at 20 μM substrate, using a protein concentration of 5 μg/ml)
  Linear with protein up to: 15 μg/ml (see note 3)(Determined at 20 μM substrate, using an incubation time of 10 min. Under these incubation conditions, the reaction is not strictly linear across the protein concentration range from 2 – 20 μg/ml: measured enzyme activity increases as the protein concentration increases)
  Vmax: 104 nmol/min/mg protein
  Km: 53 μM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: Protein Concentration: 5 mg/ml
• Storage Buffer: 10 mM sodium phosphate (pH 7.4), 140 mM sodium chloride, 20% (v/v) glycerol

Gene Information

• Gene Name: MAOB monoamine oxidase B [ Homo sapiens (human) ]
• Official Symbol: MAOB
• Synonyms: MAOB; monoamine oxidase B; amine oxidase [flavin-containing] B; MAO, brain; MAO, platelet; MAO-B; adrenalin oxidase; monoamine oxidase type B; tyramine oxidase
• Gene ID: 4129
• mRNA Refseq: NM_000898
• Protein Refseq: NP_000889
• MIM: 309860
• UniProt ID: P27338