Recombinant Human MAP1LC3B Protein, His-tagged

Cat.No. : MAP1LC3B-2412H
Product Overview : Recombinant Human MAP1LC3B Protein (Met1-Gly120) with a N-His tag was expressed in E. coli.
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Description : The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Source : E. coli
Species : Human
Tag : N-His
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 17.7 kDa
Accurate Molecular Mass: 20 kDa
Protein length : Met1-Gly120
Endotoxin : <1.0 EU per 1µg (determined by the LAL method).
Purity : > 90%
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Storage Buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
SDS-PAGE:
Gene Name : MAP1LC3B microtubule-associated protein 1 light chain 3 beta [ Homo sapiens (human) ]
Official Symbol : MAP1LC3B
Synonyms : MAP1LC3B; microtubule-associated protein 1 light chain 3 beta; microtubule-associated proteins 1A/1B light chain 3B; ATG8F; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1 light chain 3-like protein 2; autophagy-related ubiquitin-like modifier LC3 B; LC3B; MAP1LC3B-a; MAP1A/1BLC3;
Gene ID : 81631
mRNA Refseq : NM_022818
Protein Refseq : NP_073729
MIM : 609604
UniProt ID : Q9GZQ8

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