Recombinant Human MAP1LC3B Protein, His-tagged

• Cat.No.: MAP1LC3B-2412H
• Product Overview: Recombinant Human MAP1LC3B Protein (Met1-Gly120) with a N-His tag was expressed in E. coli.

Specification

• Description: The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 17.7 kDa
  Accurate Molecular Mass: 20 kDa
• Protein length: Met1-Gly120
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: MAP1LC3B microtubule-associated protein 1 light chain 3 beta [ Homo sapiens (human) ]
• Official Symbol: MAP1LC3B
• Synonyms: MAP1LC3B; microtubule-associated protein 1 light chain 3 beta; microtubule-associated proteins 1A/1B light chain 3B; ATG8F; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1 light chain 3-like protein 2; autophagy-related ubiquitin-like modifier LC3 B; LC3B; MAP1LC3B-a; MAP1A/1BLC3
• Gene ID: 81631
• mRNA Refseq: NM_022818
• Protein Refseq: NP_073729
• MIM: 609604
• UniProt ID: Q9GZQ8