Recombinant Human MAP2K2 Protein, GST-tagged, Alexa Fluor 555 conjugated

• Cat.No.: MAP2K2-968HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human MAP2K2 (NP_109587.1) (Met 1-Val 400), fused with the GST tag at the N-terminus, was produced in Baculovirus-Insect cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: GST
• Form: Lyophilized
• Molecular Mass: The recombinant human MAP2K2/GST chimera consists of 624 amino acids and has a calculated molecular mass of 70.7 kDa. It migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.
• Protein length: 624
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, pH 7.4
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: MAP2K2 mitogen-activated protein kinase kinase 2 [ Homo sapiens ]
• Official Symbol: MAP2K2
• Gene ID: 5605
• mRNA Refseq: NM_030662
• Protein Refseq: NP_109587
• MIM: 601263
• UniProt ID: P36507