Active Recombinant Human MAP2K6 protein, His&GST-tagged

• Cat.No.: MAP2K6-888H
• Product Overview: Recombinant Human MAP2K6 protein(P52564-1)(Met1-Asp334,Ser207/Asp,211Thr/Asp), fused with His and GST tag, was expressed in Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: Met1-Asp334,Ser207/Asp,211Thr/Asp
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerolPlease contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: The specific activity was determined to be ＞200 nmol/min/mg using inactive MAPK14 as substrate.
• Molecular Mass: The recombinant human MAP2K6(S207D&T211D）/GST chimera consists of 571 amino acids and has a calculated molecular mass of 65.3 KDa. It migrates as an approximately 60 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 88 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MAP2K6 mitogen-activated protein kinase kinase 6 [ Homo sapiens ]
• Official Symbol: MAP2K6
• Synonyms: MAP2K6; mitogen-activated protein kinase kinase 6; PRKMK6; dual specificity mitogen-activated protein kinase kinase 6; MAPKK6; MEK6; MKK6; protein kinase; mitogen activated; kinase 6 (MAP kinase kinase 6); SAPKK3; MEK 6; MAPKK 6; SAPK kinase 3; MAPK/ERK kinase 6; stress-activated protein kinase kinase 3; protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6); SAPKK-3
• Gene ID: 5608
• mRNA Refseq: NM_002758
• Protein Refseq: NP_002749
• MIM: 601254
• UniProt ID: P52564