Recombinant Human MAPK1, GST-tagged

• Cat.No.: MAPK1-918H
• Product Overview: Recombinant Human MAPK1 protein (Met 1-Ser 360), fused with the GST tag at the N-terminus, was expressed inInsect cells.

Specification

• Cat. No.: MAPK1-918H
• Description: Mitogen-activated protein kinase 1, also known as Extracellular signal-regulated kinase 2, ERK-2, and MAPK1, is a member of the protein kinase superfamily and MAP kinase subfamily. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. MKP-3 is a dual specificity phosphatase exclusively specific to MAPK1 for its substrate recognition and dephosphorylating activity. The activation of MAPK1 requires its phosphorylation by upstream kinases.
• Source: Insect cells.
• Molecular Mass: The recombinant human ERK2/GST chimera consists of 585 amino acids and predicts a molecular mass of 67 kDa as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met.
• Purity: > 98 % as determined by SDS-PAGE.
• Formulation: Lyophilized from sterile 50mM Tris , 100 mM Nacl, 0.5mM PMSF, 10%Glycerol, pH 8.0.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Storage And Stability: Samples are stable for up to twelve months from date of receipt at -70⁰C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: MAPK1 mitogen-activated protein kinase 1 [ Homo sapiens ]
• Synonyms: MAPK1; mitogen-activated protein kinase 1; ERK; p38; p40; p41; ERK2; ERT1; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK; MAP kinase 2; Mitogen-activated protein kinase 2; Extracellular signal-regulated kinase 2; OTTHUMP00000174492; MAP kinase 2; protein tyrosine kinase ERK2
• Gene ID: 5594
• mRNA Refseq: NM_002745
• Protein Refseq: NP_002736
• MIM: 176948
• UniProt ID: P28482
• Chromosome Location: 22q11.2; 22q11.21
• Pathway: Acute myeloid leukemia; Adherens junction; Alzheimer"s disease; Axon guidance; cell receptor signaling pathway; Bladder cancer; Chemokine signaling pathway; Chronic myeloid leukemia; Dorso-ventral axis formation; Colorectal cancer; Endometrial cancer; ErbB signaling pathway; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Focal adhesion; Gap junction; Glioma; GnRH signaling pathway; Insulin signaling pathway
• Function: ATP binding; MAP kinase 2 activity; mitogen-activated protein kinase kinase kinase binding; nucleotide binding; phosphotyrosine binding; protein binding; protein serine/threonine kinase activity; transferase activity