Recombinant Human MAPK10 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: MAPK10-4539H
• Product Overview: MAPK10 MS Standard C13 and N15-labeled recombinant protein (NP_620448) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is specifically expressed in a subset of neurons in the nervous system, and is activated by threonine and tyrosine phosphorylation. Targeted deletion of this gene in mice suggests that it may have a role in stress-induced neuronal apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 52.4 kDa
• AA Sequence: MSLHFLYYCSEPTLDVKIAFCQGFD KQVDVSYIAKHYNMSKSKVDNQFYS VEVGDSTFTVLKRYQNLKPIGSGAQ GIVCAAYDAVLDRNVAIKKLSRPFQ NQTHAKRAYRELVLMKCVNHKNIIS LLNVFTPQKTLEEFQDVYLVMELMD ANLCQVIQMELDHERMSYLLYQMLC GIKHLHSAGIIHRDLKPSNIVVKSD CTLKILDFGLARTAGTSFMMTPYVV TRYYRAPEVILGMGYKENVDIWSVG CIMGEMVRHKILFPGRDYIDQWNKV IEQLGTPCPEFMKKLQPTVRNYVEN RPKYAGLTFPKLFPDSLFPADSEHN KLKASQARDLLSKMLVIDPAKRISV DDALQHPYINVWYDPAEVEAPPPQI YDKQLDEREHTIEEWKELIYKEVMN SEEKTKNGVVKGQPSPSGAAVNSSE SLPPSSSVNDISSMSTDQTLASDTD SSLEASAGPLGCCRTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: MAPK10 mitogen-activated protein kinase 10 [ Homo sapiens (human) ]
• Official Symbol: MAPK10
• Synonyms: MAPK10; mitogen-activated protein kinase 10; PRKM10; JNK3; p54bSAPK; p493F12; MAPK 10; MAP kinase 10; MAP kinase p49 3F12; JNK3 alpha protein kinase; c-Jun N-terminal kinase 3; stress-activated protein kinase 1b; stress activated protein kinase beta; stress-activated protein kinase JNK3; JNK3A; SAPK1b; FLJ12099; FLJ33785; MGC50974
• Gene ID: 5602
• mRNA Refseq: NM_138982
• Protein Refseq: NP_620448
• MIM: 602897
• UniProt ID: P53779