Recombinant Human MAPT 293 Cell Lysate

Cat.No. : MAPT-4477HCL
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Description : Antigen standard for microtubule-associated protein tau (MAPT), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag : Non
Gene Name : MAPT microtubule-associated protein tau [ Homo sapiens ]
Official Symbol : MAPT
Synonyms : MAPT; microtubule-associated protein tau; DDPAC, MAPTL; FLJ31424; FTDP 17; G protein beta1/gamma2 subunit interacting factor 1; MGC138549; microtubule associated protein tau; isoform 4; MSTD; MTBT1; MTBT2; PPND; tau; TAU; PHF-tau; paired helical filament-tau; neurofibrillary tangle protein; microtubule-associated protein tau, isoform 4; G protein beta1/gamma2 subunit-interacting factor 1; DDPAC; MAPTL; FTDP-17;
Gene ID : 4137
mRNA Refseq : NM_016834
Protein Refseq : NP_058518
MIM : 157140
UniProt ID : P10636
Chromosome Location : 17q21
Pathway : Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cellular proteins, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; Caspase-mediated cleavage of cytoskeletal proteins, organism-specific biosystem; IL-6 Signaling Pathway, organism-specific biosystem;
Function : SH3 domain binding; apolipoprotein E binding; enzyme binding; lipoprotein particle binding; microtubule binding; protein binding; structural constituent of cytoskeleton;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Customer Reviews (3)

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Reviews
07/22/2022

    Western-blot experiments using MAPT as a target have shown highly reproducible results.

    08/08/2020

      This stability of MAPT is very good and can maintain its original properties even after multiple uses.

      09/15/2019

        MAPT's purity and consistency make it a top choice for Western-blot experiments.

        Q&As (6)

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        How to assess the degree of abnormal aggregation of MAPT protein? 12/13/2019

        The degree of abnormal aggregation of MAMAP proteins can be assessed by a variety of methods, such as immunohistochemistry, immunofluorescence staining, Western blot, etc. These methods can detect and quantify abnormally aggregated MAPT proteins.

        What is the relationship between MAPT protein and nerve cytoskeleton? 11/30/2019

        The MAPT protein is closely related to the nerve cytoskeleton. It is involved in regulating axon formation and maintenance, cytosolic flow, and the normal function of neurons by binding and stabilizing microtubules.

        What are the interactions of the MAPT protein with other proteins in the nervous system? 10/24/2019

        MAPT protein interacts with other proteins in the nervous system, such as α-synuclein, β-kinigin, etc. These interactions may be involved in regulating the structure and function of neurons.

        Is there a mechanism that regulates the expression of MAPT protein? 10/21/2019

        Yes, the expression of MAPT protein is affected by a variety of regulatory mechanisms, including transcriptional regulation, splicing regulation, phosphorylation regulation, etc. These regulatory mechanisms are essential for maintaining normal MAPT protein levels and function.

        What are the future directions for MAPT protein research? 07/06/2019

        Future research directions include further elucidating the mechanism of abnormal aggregation and phosphorylation of MAPT protein, exploring the pathogenesis of genetic diseases associated with MAPT gene, finding therapeutic strategies to intervene in abnormal aggregation of MATT, and developing biomarkers related to MAPT protein for early diagnosis and therapeutic monitoring.

        How about the applications of MAPT protein in clinical diagnosis? 07/05/2019

        The MAPT protein plays an important role in clinical diagnosis. By measuring the level of the MAPT protein in the blood or cerebrospinal fluid, it can help identify different types of neurodegenerative diseases, such as Alzheimer's disease and prefrontal temporal dementia.

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