Recombinant Human MAPT 293 Cell Lysate
Cat.No. : | MAPT-4477HCL |
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Description : | Antigen standard for microtubule-associated protein tau (MAPT), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Tag : | Non |
Gene Name : | MAPT microtubule-associated protein tau [ Homo sapiens ] |
Official Symbol : | MAPT |
Synonyms : | MAPT; microtubule-associated protein tau; DDPAC, MAPTL; FLJ31424; FTDP 17; G protein beta1/gamma2 subunit interacting factor 1; MGC138549; microtubule associated protein tau; isoform 4; MSTD; MTBT1; MTBT2; PPND; tau; TAU; PHF-tau; paired helical filament-tau; neurofibrillary tangle protein; microtubule-associated protein tau, isoform 4; G protein beta1/gamma2 subunit-interacting factor 1; DDPAC; MAPTL; FTDP-17; |
Gene ID : | 4137 |
mRNA Refseq : | NM_016834 |
Protein Refseq : | NP_058518 |
MIM : | 157140 |
UniProt ID : | P10636 |
Chromosome Location : | 17q21 |
Pathway : | Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cellular proteins, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; Caspase-mediated cleavage of cytoskeletal proteins, organism-specific biosystem; IL-6 Signaling Pathway, organism-specific biosystem; |
Function : | SH3 domain binding; apolipoprotein E binding; enzyme binding; lipoprotein particle binding; microtubule binding; protein binding; structural constituent of cytoskeleton; |
Products Types
◆ Recombinant Protein | ||
MAPT-516H | Active Recombinant Human MAPT Protein | +Inquiry |
MAPT-2886H | Recombinant Human MAPT protein(1-441 aa, Tau181), C-His-tagged | +Inquiry |
MAPT-654R | Recombinant Rhesus macaque MAPT protein(2-459aa), His-tagged | +Inquiry |
MAPT-032H | Recombinant Human MAPT Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
MAPT-22H | Recombinant Human SAMHD1 Protein, Myc/DDK-tagged | +Inquiry |
◆ Lysates | ||
MAPT-4478HCL | Recombinant Human MAPT 293 Cell Lysate | +Inquiry |
MAPT-4476HCL | Recombinant Human MAPT 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewWestern-blot experiments using MAPT as a target have shown highly reproducible results.
This stability of MAPT is very good and can maintain its original properties even after multiple uses.
MAPT's purity and consistency make it a top choice for Western-blot experiments.
Q&As (6)
Ask a questionThe degree of abnormal aggregation of MAMAP proteins can be assessed by a variety of methods, such as immunohistochemistry, immunofluorescence staining, Western blot, etc. These methods can detect and quantify abnormally aggregated MAPT proteins.
The MAPT protein is closely related to the nerve cytoskeleton. It is involved in regulating axon formation and maintenance, cytosolic flow, and the normal function of neurons by binding and stabilizing microtubules.
MAPT protein interacts with other proteins in the nervous system, such as α-synuclein, β-kinigin, etc. These interactions may be involved in regulating the structure and function of neurons.
Yes, the expression of MAPT protein is affected by a variety of regulatory mechanisms, including transcriptional regulation, splicing regulation, phosphorylation regulation, etc. These regulatory mechanisms are essential for maintaining normal MAPT protein levels and function.
Future research directions include further elucidating the mechanism of abnormal aggregation and phosphorylation of MAPT protein, exploring the pathogenesis of genetic diseases associated with MAPT gene, finding therapeutic strategies to intervene in abnormal aggregation of MATT, and developing biomarkers related to MAPT protein for early diagnosis and therapeutic monitoring.
The MAPT protein plays an important role in clinical diagnosis. By measuring the level of the MAPT protein in the blood or cerebrospinal fluid, it can help identify different types of neurodegenerative diseases, such as Alzheimer's disease and prefrontal temporal dementia.
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