Recombinant Human MAPT

• Cat.No.: MAPT-94H
• Product Overview: Recombinant human tag-free Tau-441 was co-expressed with BRSK1 in E. coli cells. The Tau-441 was phosphorylated by BRSK1 in vivo and in vitro prior to the final chromatography purification.

Specification

• Description: This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer"s disease, Pick"s disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
• Source: E.coli
• Species: Human
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~64 kDa
• AA Sequence: 1-441
• Purity: The purity of Tau441, BRSK1-phosphorylated was determined to be >80% by densitometry, approx.
• Applications: Kinase Assay; Western Blot
• Stability: 1 yr at –70oC from date of shipment.
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.2 ug/ul
• Tag: Non

Gene Information

• Gene Name: MAPT?microtubule-associated protein tau [?Homo sapiens?(human) ]
• Official Symbol: MAPT
• Synonyms: MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; microtubule-associated protein tau; microtubule-associated protein tau; PHF-tau; paired helical filament-tau; neurofibrillary tangle protein; microtubule-associated protein tau, isoform 4; G protein beta1/gamma2 subunit-interacting factor 1
• Gene ID: 4137
• mRNA Refseq: NM_005910
• Protein Refseq: NP_005901??
• MIM: 157140
• UniProt ID: P10636
• Chromosome Location: 17q21.1
• Pathway: Alzheimer"s disease; Caspase-mediated cleavage of cytoskeletal proteins; IL-2 Signaling pathway
• Function: SH3 domain binding; apolipoprotein binding; enzyme binding

Reviews

07/22/2022

Western-blot experiments using MAPT as a target have shown highly reproducible results.

08/08/2020

This stability of MAPT is very good and can maintain its original properties even after multiple uses.

09/15/2019

MAPT's purity and consistency make it a top choice for Western-blot experiments.

Q&As

How to assess the degree of abnormal aggregation of MAPT protein?

The degree of abnormal aggregation of MAMAP proteins can be assessed by a variety of methods, such as immunohistochemistry, immunofluorescence staining, Western blot, etc. These methods can detect and quantify abnormally aggregated MAPT proteins.

What is the relationship between MAPT protein and nerve cytoskeleton?

The MAPT protein is closely related to the nerve cytoskeleton. It is involved in regulating axon formation and maintenance, cytosolic flow, and the normal function of neurons by binding and stabilizing microtubules.

What are the interactions of the MAPT protein with other proteins in the nervous system?

MAPT protein interacts with other proteins in the nervous system, such as α-synuclein, β-kinigin, etc. These interactions may be involved in regulating the structure and function of neurons.

Is there a mechanism that regulates the expression of MAPT protein?

Yes, the expression of MAPT protein is affected by a variety of regulatory mechanisms, including transcriptional regulation, splicing regulation, phosphorylation regulation, etc. These regulatory mechanisms are essential for maintaining normal MAPT protein levels and function.

What are the future directions for MAPT protein research?

Future research directions include further elucidating the mechanism of abnormal aggregation and phosphorylation of MAPT protein, exploring the pathogenesis of genetic diseases associated with MAPT gene, finding therapeutic strategies to intervene in abnormal aggregation of MATT, and developing biomarkers related to MAPT protein for early diagnosis and therapeutic monitoring.

How about the applications of MAPT protein in clinical diagnosis?

The MAPT protein plays an important role in clinical diagnosis. By measuring the level of the MAPT protein in the blood or cerebrospinal fluid, it can help identify different types of neurodegenerative diseases, such as Alzheimer's disease and prefrontal temporal dementia.