Recombinant Human MARCKS Protein, His&SUMO-tagged

• Cat.No.: MARCKS-1097H
• Product Overview: Recombinant Human MARCKS Protein (Gly2-Glu332) with His&SUMO tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
• Source: E. coli
• Species: Human
• Tag: N-His&SUMO
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 45.1 kDa; Accurate Molecular Mass: 40 kDa
• Protein length: Gly2-Glu332
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: MARCKS myristoylated alanine-rich protein kinase C substrate [ Homo sapiens (human) ]
• Official Symbol: MARCKS
• Synonyms: MARCKS; myristoylated alanine-rich protein kinase C substrate; MACS, myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L); myristoylated alanine-rich C-kinase substrate; 80K L; PKCSL; phosphomyristin; protein kinase C substrate, 80 kDa protein, light chain; myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L); MACS; 80K-L; PRKCSL; FLJ14368; FLJ90045
• Gene ID: 4082
• mRNA Refseq: NM_002356
• Protein Refseq: NP_002347
• MIM: 177061
• UniProt ID: P29966