Recombinant Human MARS, MYC/DDK-tagged

• Cat.No.: MARS-20H
• Product Overview: Recombinant Human MARS, fused with C-terminal MYC/DDK, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules.
• Molecular Mass: 100.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: MARS methionyl-tRNA synthetase [ Homo sapiens (human) ]
• Official Symbol: MARS
• Synonyms: MARS; MRS; METRS; MTRNS; SPG70; methionyl-tRNA synthetase; methionine--tRNA ligase, cytoplasmic; cytosolic methionyl-tRNA synthetase; NP_004981.2; EC 6.1.1.10
• Gene ID: 4141
• mRNA Refseq: NM_004990
• Protein Refseq: NP_004981
• MIM: 156560
• UniProt ID: P56192
• Chromosome Location: 12q13
• Pathway: Aminoacyl-tRNA biosynthesis; Cytosolic tRNA aminoacylation; Selenocompound metabolism
• Function: ATP binding; methionine-tRNA ligase activity; tRNA binding

Citation

SMPDL3b modulates insulin receptor signaling in diabetic kidney disease

Journal: Nature Communications    PubMed ID: 31217420    Data: 2019/6/19

Authors: A. Mitrofanova, S. K. Mallela, A. Fornoni

Article Snippet:C16:0 C1P (#860533) and C6-NBD-Ceramide (#810209P) were obtained from Avanti Polar Lipid (AL, USA).C16:0 C1P (#860533) and C6-NBD-Ceramide (#810209P) were obtained from Avanti Polar Lipid (AL, USA).. Recombinant human SMPDL3b protein, MYC/DDK-tagged was ordered from Creative BioMart (#SMPDL3b-2823H).. Anti-caveolin-1 (#3251 for phospho-Cav1(Tyr14), dilution 1:1000, #3267 for Cav1 (D46G3) XP TM , dilution 1:1000), anti-IR β subunit (4B8) (#3025, dilution 1:100), anti-Na/K-ATPase (#3010, dilution 1:1000), anti-MEK-1/2 (D1A5) (#8727, dilution 1:1,000), anti-AKT (#9271 for phospho-AKT (Ser473) (D9E), dilution 1:1000, #9272 for tAKT, dilution 1:1000), anti-p70S6Ke (#9234 for phosphor-p70S6K (Thr389 (108D2)), dilution 1:1000, #9202 for p70S6K, dilution 1:1000), anti-4EBP1 (#2855 for phospho-4E-BP1 (Thr37/46) (236B4), dilution 1:1000, #9644 for 4E-BP1, dilution 1:1000) primary antibodies were purchased from Cell Signaling Technology (MA, USA).Anti-caveolin-1 (#3251 for phospho-Cav1(Tyr14), dilution 1:1000, #3267 for Cav1 (D46G3) XP TM , dilution 1:1000), anti-IR β subunit (4B8) (#3025, dilution 1:100), anti-Na/K-ATPase (#3010, dilution 1:1000), anti-MEK-1/2 (D1A5) (#8727, dilution 1:1,000), anti-AKT (#9271 for phospho-AKT (Ser473) (D9E), dilution 1:1000, #9272 for tAKT, dilution 1:1000), anti-p70S6Ke (#9234 for phosphor-p70S6K (Thr389 (108D2)), dilution 1:1000, #9202 for p70S6K, dilution 1:1000), anti-4EBP1 (#2855 for..