Recombinant Human Matrix Metallopeptidase 8

• Cat.No.: MMP8-41H
• Product Overview: Recombinant Human Matrix Metalloproteinase-8 produced inE.Coliis a single, non-glycosylated, polypeptide chain having a molecular mass of 75 kDa. The MMP-8 is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Full-length recombinant human neutrophil pro-collagenase (MMP-8), latent form. Matrix metalloproteinase 8 (MMP-8), or neutrophil collagenase, degrades interstitial collagens, acting preferentially on collagen type I. Increased full-length MMP-8 protein was associated with infiltration into the skin of neutrophils, which are the major cell type that expresses MMP-8. MMP-8 is synthesized and stored in specific granules in neutrophil leukocytes. MMP-8 activity is therefore regulated by factors such as surface-bound ligands (IgG or complement components) that release it through degranulation.Once released and activated through proteolytic or oxidative mechanisms, MMP-8 plays a major role in the connective tissue turnover that accompanies inflammatory processes.
• Physical Appearance: Sterile Filtered clear solution.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Formulation: The protein Solution (100 units/ml) in 0.05 M Tris-HCl buffer, pH 7.6, containing 0.2 M NaCl, 5 mM CaCl2, 0.0025% NaN3 and 0.1% BSA.
• Biological Activity: 100 units/ml after activation with APMA by solution assay method. One unit of collagenolytic activity is defined as the cleavage of 1µg of collagen per minute by the solution method.
• Applications: Used as a standard for analyzing mammalian collagenase activity.
• Stability: MMP-8 although stable at 14°C for 1 week, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: MMP8 matrix metallopeptidase 8 (neutrophil collagenase) [ Homo sapiens ]
• Synonyms: MMP8; matrix metallopeptidase 8 (neutrophil collagenase); HNC; CLG1; PMNL-CL; PMNL collagenase; matrix metalloproteinase 8; EC3.4.24.34; MMP-8; PMNL-CL; PMNL collagenase; Matrix metalloproteinase-8
• Gene ID: 4317
• mRNA Refseq: NM_002424
• Protein Refseq: NP_002415
• MIM: 120355
• UniProt ID: P22894
• Chromosome Location: 11q22.3
• Function: calcium ion binding; metalloendopeptidase activity; peptidase activity; serine-type endopeptidase activity; zinc ion binding