Recombinant Human MBD1 Protein, His-tagged

• Cat.No.: MBD1-2425H
• Product Overview: Recombinant Human MBD1 Protein (Met1-Leu216) with a N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 27.6 kDa
  Accurate Molecular Mass: 28 kDa
• Protein length: Met1-Leu216
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: MBD1 methyl-CpG binding domain protein 1 [ Homo sapiens (human) ]
• Official Symbol: MBD1
• Synonyms: MBD1; methyl-CpG binding domain protein 1; methyl-CpG-binding domain protein 1; CXXC3; PCM1; CXXC-type zinc finger protein 3; protein containing methyl-CpG-binding domain 1; methyl-CpG binding domain protein 1 isoform PCM1; the regulator of fibroblast growth factor 2 (FGF-2) transcription; RFT
• Gene ID: 4152
• mRNA Refseq: NM_001204136
• Protein Refseq: NP_001191065
• MIM: 156535
• UniProt ID: Q9UIS9