Recombinant Human MBP Protein, His&SUMO-tagged

• Cat.No.: MBP-1100H
• Product Overview: Recombinant Human MBP Protein (Ala135-Ser190) with His&SUMO tag was expressed in E. coli.

Specification

• Description: The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
• Source: E. coli
• Species: Human
• Tag: His&SUMO
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 19.8 kDa; Accurate Molecular Mass: 22 kDa
• Protein length: Ala135-Ser190
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: MBP myelin basic protein [ Homo sapiens (human) ]
• Official Symbol: MBP
• Synonyms: MBP; myelin basic protein; Golli-MBP; myelin basic protein; myelin A1 protein; myelin membrane encephalitogenic protein; MGC99675
• Gene ID: 4155
• mRNA Refseq: NM_001025081
• Protein Refseq: NP_001020252
• MIM: 159430
• UniProt ID: P02686

Reviews

12/21/2019

Product exactly as described.

07/27/2018

Worked well in ELISA.

05/13/2018

Excellent customer support, helpful.

Q&As

What is the primary function of the MBP gene and its encoded protein?

The MBP gene encodes the Myelin Basic Protein, which is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system.

How is MBP expression regulated during neural development?

MBP expression is tightly regulated during neural development. It increases as oligodendrocytes differentiate and begin to form the myelin sheath around axons.

What role does MBP play in multiple sclerosis (MS)?

In multiple sclerosis (MS), an autoimmune disease, MBP is one of the targets for immune cells. The immune system attacks and degrades the myelin sheath, leading to the characteristic symptoms of MS.

What are the implications of MBP in demyelinating diseases beyond MS?

Other than MS, demyelinating diseases like Pelizaeus-Merzbacher disease and some leukodystrophies can be linked to issues with MBP. These conditions can lead to a range of neurological symptoms due to impaired nerve transmission.

Are there any therapeutic strategies targeting MBP or its pathways in neurodegenerative diseases?

Therapeutic strategies targeting MBP mainly focus on preventing the immune system from attacking it, especially in the context of MS. This includes the development of immunomodulatory drugs and therapies to promote remyelination.

Are there any known diseases or conditions associated with mutations or dysregulation of MBP?

Beyond MS, mutations or dysregulation of MBP can lead to other rare demyelinating diseases and some forms of leukodystrophy.

How does MBP contribute to the structure and function of myelin sheaths in the nervous system?

MBP plays a crucial role in the formation of the myelin sheath, a fatty layer that insulates nerve fibers. It helps compact and stabilize the layers of myelin, ensuring efficient nerve signal transmission.