||Recombinant Human MCCC1 fused with His tag at N-terminal was expressed in E. coli.
||This gene encodes the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.
||25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1 % Sarkosyl.
||> 80% as determined by SDS-PAGE and Coomassie blue staining
||>50 ug/mL as determined by microplate BCA method