Recombinant Human MCM3, His-tagged

• Cat.No.: MCM3-28038TH
• Product Overview: Recombinant fragment, corresponding to amino acids 182-808 of Human MCM3 with an N-terminal His tag; Predicted MWt 71kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 182-808 a.a.
• Description: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.
• Conjugation: HIS
• Form: Lyophilised:Reconstitute with 84 μl aqua dest
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: NPLETEYGLSVYKDHQTITIQEMPEKAPAGQLPRSVDVIL DDDLVDKAKPGDRVQVVGTYRCLPGKKGGYTSGTFRTV LIACNVKQMSKDAQPSFSAEDIAKIKKFSKTRSKDIFD QLAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHI RGDINILLIGDPSVAKSQLLRYVLCTAPRAIPTTGRGSSG VGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDK MSDMDRTAIHEVMEQGRVTIAKAGIHARLNARCSVLAA ANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQM DPEQDREISDHVLRMHRYRAPGEQDGDAMPLGSAVDILAT DDPNFSQEDQQDTQIYEKHDNLLHGTKKKKEKMVSAAF MKKYIHVAKIIKPVLTQESATYIAEEYSRLRSQDSMSS DTARTSPVTARTLETLIRLATAHAKARMSKTVDLQDAE EAVELVQYAYFKKVLEKEKKRKKRSEDESETEDEEEKSQEDQEQKRKRRKTRQPDAKDGDSYDPYDFSDTEEEMPQVH TPKTADSQETKESQKVELSESRLKAFKVALLDVFREAH AQSIGMNRLTESINRDSEEPFSSVEIQAALSKMQDDNQ VMVSEGIIFLI
• Sequence Similarities: Belongs to the MCM family.Contains 1 MCM domain.

Gene Information

• Gene Name: MCM3 minichromosome maintenance complex component 3 [ Homo sapiens ]
• Official Symbol: MCM3
• Synonyms: MCM3; minichromosome maintenance complex component 3; MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) , minichromosome maintenance deficient (S. cerevisiae) 3; DNA replication licensing factor MCM3
• Gene ID: 4172
• mRNA Refseq: NM_002388
• Protein Refseq: NP_002379
• MIM: 602693
• Uniprot ID: P25205
• Chromosome Location: 6p12
• Pathway: Activation of ATR in response to replication stress, organism-specific biosystem; Activation of the pre-replicative complex, organism-specific biosystem; Assembly of the pre-replicative complex, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem
• Function: ATP binding; DNA binding; helicase activity; hydrolase activity; nucleotide binding