Recombinant Human ME1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ME1-5947H
• Product Overview: ME1 MS Standard C13 and N15-labeled recombinant protein (NP_002386) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 64.1 kDa
• AA Sequence: MEPEAPRRRHTHQRGYLLTRNPHLN KDLAFTLEERQQLNIHGLLPPSFNS QEIQVLRVVKNFEHLNSDFDRYLLL MDLQDRNEKLFYRVLTSDIEKFMPI VYTPTVGLACQQYSLVFRKPRGLFI TIHDRGHIASVLNAWPEDVIKAIVV TDGERILGLGDLGCNGMGIPVGKLA LYTACGGMNPQECLPVILDVGTENE ELLKDPLYIGLRQRRVRGSEYDDFL DEFMEAVSSKYGMNCLIQFEDFANV NAFRLLNKYRNQYCTFNDDIQGTAS VAVAGLLAALRITKNKLSDQTILFQ GAGEAALGIAHLIVMALEKEGLPKE KAIKKIWLVDSKGLIVKGRASLTQE KEKFAHEHEEMKNLEAIVQEIKPTA LIGVAAIGGAFSEQILKDMAAFNER PIIFALSNPTSKAECSAEQCYKITK GRAIFASGSPFDPVTLPNGQTLYPG QGNNSYVFPGVALGVVACGLRQITD NIFLTTAEVIAQQVSDKHLEEGRLY PPLNTIRDVSLKIAEKIVKDAYQEK TATVYPEPQNKEAFVRSQMYSTDYD QILPDCYSWPEEVQKIQTKVDQTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ME1 malic enzyme 1 [ Homo sapiens (human) ]
• Official Symbol: ME1
• Synonyms: ME1; malic enzyme 1, NADP(+)-dependent, cytosolic; NADP-dependent malic enzyme; NADP-ME; malate dehydrogenase; malic enzyme 1, soluble; Malic enzyme, cytoplasmic; pyruvic-malic carboxylase; MES; HUMNDME
• Gene ID: 4199
• mRNA Refseq: NM_002395
• Protein Refseq: NP_002386
• MIM: 154250
• UniProt ID: P48163