Recombinant Human MED15 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: MED15-4823H
• Product Overview: MED15 MS Standard C13 and N15-labeled recombinant protein (NP_056973) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Alternative splicing results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 82.6 kDa
• AA Sequence: MDVSGQETDWRSTAFRQKLVSQIED AMRKAGVAHSKSSKDMESHVFLKAK TRDEYLSLVARLIIHFRDIHNKKSQ ASVSDPMNALQSLTGGPAAGAAGIG MPPRGPGQSLGGMGSLGAMGQPMSL SGQPPPGTSGMAPHSMAVVSTATPQ TQLQLQQVALQQQQQQQQFQQQQQA ALQQQQQQQQQQQFQAQQSAMQQQF QAVVQQQQQLQQQQQQQQHLIKLHH QNQQQIQQQQQQLQRIAQLQLQQQQ QQQQQQQQQQQQALQAQPPIQQPPM QQPQPPPSQALPQQLQQMHHTQHHQ PPPQPQQPPVAQNQPSQLPPQSQTQ PLVSQAQALPGQMLYTQPPLKFVRA PMVVQQPPVQPQVQQQQTAVQTAQA AQMVAPGVQVSQSSLPMLSSPSPGQ QVQTPQSMPPPPQPSPQPGQPSSQP NSNVSSGPAPSPSSFLPSPSPQPSQ SPVTARTPQNFSVPSPGPLNTPVNP SSVMSPAGSSQAEEQQYLDKLKQLS KYIEPLRRMINKIDKNEDRKKDLSK MKSLLDILTDPSKRCPLKTLQKCEI ALEKLKNDMAVPTPPPPPVPPTKQQ YLCQPLLDAVLANIRSPVFNHSLYR TFVPAMTAIHGPPITAPVVCTRKRR LEDDERQSIPSVLQGEVARLDPKFL VNLDPSHCSNNGTVHLICKLDDKDL PSVPPLELSVPADYPAQSPLWIDRQ WQYDANPFLQSVHRCMTSRLLQLPD KHSVTALLNTWAQSVHQACLSAATR TRPLEQKLISEEDLAANDILDYKDD DDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: MED15 mediator complex subunit 15 [ Homo sapiens (human) ]
• Official Symbol: MED15
• Synonyms: MED15; mediator complex subunit 15; PC2 (positive cofactor 2, multiprotein complex) glutamine/Q rich associated protein, PCQAP, TNRC7, trinucleotide repeat containing 7; mediator of RNA polymerase II transcription subunit 15; Arc105; CAG7A; TIG 1; TPA inducible gene-1; CTG repeat protein 7a; TPA inducible protein; TPA-inducible gene 1 protein; trinucleotide repeat containing 7; activator-recruited cofactor, 105-kD; PC2-glutamine-rich-associated protein; PC2 glutamine/Q-rich-associated protein; activator-recruited cofactor 105 kDa component; trinucleotide repeat-containing gene 7 protein; positive cofactor 2 glutamine/Q-rich-associated protein; positive cofactor 2, glutamine/Q-rich-associated protein; PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein; TIG1; CTG7A; PCQAP; TIG-1; TNRC7; ARC105; FLJ42282; FLJ42935; DKFZp686A2214; DKFZp762B1216
• Gene ID: 51586
• mRNA Refseq: NM_015889
• Protein Refseq: NP_056973
• MIM: 607372
• UniProt ID: Q96RN5