Recombinant Human MED17 protein, His-tagged

• Cat.No.: MED17-2800H
• Product Overview: Recombinant Human MED17 protein(296-651 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 296-651 aa
• AA Sequence: EAAQNVLLCKEIFAQLSREAVQIKS QVPHIVVKNQIISQPFPSLQLSISL CHSSNDKKSQKFATEKQCPEDHLYV LEHNLHLLIREFHKQTLSSIMMPHP ASAPFGHKRMRLSGPQAFDKNEINS LQSSEGLLEKIIKQAKHIFLRSRAA ATIDSLASRIEDPQIQAHWSNINDV YESSVKVLITSQGYEQICKSIQLQL NIGVEQIRVVHRDGRVITLSYQEQE LQDFLLSQMSQHQVHAVQQLAKVMG WQVLSFSNHVGLGPIESIGNASAIT VASPSGDYAISVRNGPESGSKIMVQ FPRNQCKDLPKSDVLQDNKWSHLRG PFKEVQWNKMEGRNFVYKMELLMSA LSPCLL
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: MED17 mediator complex subunit 17 [ Homo sapiens ]
• Official Symbol: MED17
• Synonyms: MED17; mediator complex subunit 17; cofactor required for Sp1 transcriptional activation, subunit 6 (77kD) , cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa , CRSP6; mediator of RNA polymerase II transcription subunit 17; CRSP77; DRIP80; TRAP80; ARC77; CRSP complex subunit 6; transcriptional coactivator CRSP77; activator-recruited cofactor 77 kDa component; vitamin D3 receptor-interacting protein complex 80 kDa component; thyroid hormone receptor-associated protein complex 80 kDa component; cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa; CRSP6; FLJ10812
• Gene ID: 9440
• mRNA Refseq: NM_004268
• Protein Refseq: NP_004259
• MIM: 603810
• UniProt ID: Q9NVC6