Recombinant Human Megakaryocyte-Associated Tyrosine Kinase, His-tagged

• Cat.No.: MATK-5451H
• Product Overview: Recombinant humanfull length MATK, His-tagged. No special measures were taken to activate thiskinase.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: MATK encoded bythis gene has amino acid sequence similarity to Csk tyrosine kinase and hasthe structural features of the CSK subfamily: SRC homology SH2 and SH3domains, a catalytic domain, a unique N terminus, lack of myristylationsignals, lack of a negative regulatory phosphorylation site, and lack of anautophosphorylation site. This protein is thought to play a significant rolein the signal transduction of hematopoietic cells. It is able tophosphorylate and inactivate Src family kinases, and may play an inhibitoryrole in the control of T-cell proliferation. This protein might be involvedin signaling in some cases of breast cancer. Three alternatively splicedtranscript variants that encode different isoforms have been described forthis gene.
• Form: Liquid in 20 mMHEPES, pH 7.5 + 150 mM NaCl + 0.5 mM EDTA + 0.5 mM EGTA + 0.05% CHAPS + 5 mMDTT + 50% glycerol
• Molecular Weight: 53.0 kDa
• Concentration: 0.4 mg/ml
• Purity: ≥ 90% as determinedby SDS-PAGE analysis
• Specific Activity: 490 nmole ofphosphate transferred to poly [Glu,Tyr] 4:1 substrate per minute per mg oftotal protein at 30°C. Activity determined at a final protein concentrationof 4 µg/ml.
• Storage: Stable for 6 monthsin working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: MATK
• Full Length: Full L.

Gene Information

• Gene Name: MATKmegakaryocyte-associated tyrosine kinase [ Homo sapiens ]
• Synonyms: MATK;megakaryocyte-associated tyrosine kinase; CHK; CTK; HYL; Lsk; HYLTK; HHYLTK;MGC1708; MGC2101; DKFZp434N1212; megakaryocyte-associated tyrosine-proteinkinase; protein kinase HYL; HYL tyrosine kinase; tyrosine kinase MATK;Csk-homologous kinase; tyrosylprotein kinase; hydroxyaryl-protein kinase; tyrosine-proteinkinase CTK; Csk-type protein tyrosine kinase; leukocyte carboxyl-terminal srckinase related; hematopoietic consensus tyrosine-lacking kinase; EC 2.7.10.2
• Gene ID: 4145
• mRNA Refseq: NM_139354
• Protein Refseq: NP_647611
• MIM: 600038
• UniProt ID: P42679
• Chromosome Location: 19p13.3
• Pathway: IL-3 SignalingPathway; Kit Receptor Signaling Pathway; Neurotrophic factor-mediated Trkreceptor signaling; Neurotrophin signaling pathway; Signaling events mediatedby Stem cell factor receptor (c-Kit)
• Function: ATP binding;non-membrane spanning protein tyrosine kinase activity; nucleotide binding;protein tyrosine kinase activity