Recombinant Human MET protein, His-Avi-tagged

• Cat.No.: MET-105H
• Product Overview: Recombinant Human HGFR/c-MET Protein is expressed from mammalian with a poly-His and Avi tag at the C-terminal. It contains Glu 25 - Thr 932.

Specification

• Description: c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene.The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling.
• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Supplied as 0.22um filtered solution in PBS (pH 7.4).
• Molecular Mass: The Human HGFR/c-MET Protein has a calculated MW of 104.6 kDa .As a result of glycosylation, the protein migrates as 80-85 kDa under reducing (R) condition (Bis-Tris PAGE)
• Protein length: It contains Glu 25 - Thr 932 .
• Endotoxin: Less than 0.05 EU per ug by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: MET met proto-oncogene (hepatocyte growth factor receptor) [ Homo sapiens ]
• Official Symbol: MET
• Synonyms: MET; oncogene MET; Proto-oncogene c-Met ;HGF R; HGF receptor; HGF/SF receptor; HGFR;AUTS9; cMET; c-MET; EC 2.7.10; EC 2.7.10.1; hepatocyte growth factor receptor; Met (c-Met); met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met
• Gene ID: 4233
• mRNA Refseq: NM_000245
• Protein Refseq: NP_000236
• MIM: 164860
• UniProt ID: P08581