Recombinant Human MIB1 protein, His & T7-tagged

• Cat.No.: MIB1-1883H
• Product Overview: Recombinant Human MIB1 aa. (Arg769~Glu1000 (Accession # Q86YT6)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1).
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.4kDa
• Protein length: Arg769~Glu1000 (Accession # Q86YT6)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 8.3

Gene Information

• Gene Name: MIB1 mindbomb E3 ubiquitin protein ligase 1 [ Homo sapiens (human) ]
• Official Symbol: MIB1
• Synonyms: MIB1; mindbomb E3 ubiquitin protein ligase 1; MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2; E3 ubiquitin-protein ligase MIB1; DAPK-interacting protein 1; RING-type E3 ubiquitin transferase MIB1; ubiquitin ligase mind bomb; zinc finger ZZ type with ankyrin repeat domain protein 2
• Gene ID: 57534
• mRNA Refseq: NM_020774.3
• Protein Refseq: NP_065825.1
• UniProt ID: Q86YT6