Active Recombinant Full Length Human MIB1 Protein, C-Flag-tagged

• Cat.No.: MIB1-124HFL
• Product Overview: Recombinant Full Length Human MIB1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1).
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: In vitro ubiquitination assay
• Molecular Mass: 110 kDa
• AA Sequence: MSNSRNNRVMVEGVGARVVRGPDWK WGKQDGGEGHVGTVRSFESPEEVVV VWDNGTAANYRCSGAYDLRI LDSAPTGIKHDGTMCDTCRQQPIIG IRWKCAECTNYDLCTVCYHGDKHHL RHRFYRITTPGSERVLLESR RKSKKITARGIFAGARVVRGVDWQW EDQDGGNGRRGKVTEIQDWSASSPH SAAYVLWDNGAKNLYRVGFE GMSDLKCVQDAKGGSFYRDHCPVLG EQNGNRNPGGLQIGDLVNIDLDLEI VQSLQHGHGGWTDGMFETLT TTGTVCGIDEDHDIVVQYPSGNRWT FNPAVLTKANIVRSGDAAQGAEGGT SQFQVGDLVQVCYDLERIKL LQRGHGEWAEAMLPTLGKVGRVQQI YSDSDLKVEVCGTSWTYNPAAVSKV ASAGSAISNASGERLSQLLK KLFETQESGDLNEELVKAAANGDVA KVEDLLKRPDVDVNGQCAGHTAMQA ASQNGHVDILKLLLKQNVDV EAEDKDGDRAVHHAAFGDEGAVIEV LHRGSADLNARNKRRQTPLHIAVNK GHLQVVKTLLDFGCHPSLQD SEGDTPLHDAISKKRDDILAVLLEA GADVTITNNNGFNALHHAALRGNPS AMRVLLSKLPRPWIVDEKKD DGYTALHLAALNNHVEVAELLVHQG NANLDIQNVNQQTALHLAVERQHTQ IVRLLVRAGAKLDIQDKDGD TPLHEALRHHTLSQLRQLQDMQDVG KVDAAWEPSKNTLIMGLGTQGAEKK SAASIACFLAANGADLSIRN KKGQSPLDLCPDPNLCKALAKCHKE KVSGQVGSRSPSMISNDSETLEECM VCSDMKRDTLFGPCGHIATC SLCSPRVKKCLICKEQVQSRTKIEE CVVCSDKKAAVLFQPCGHMCACENC ANLMKKCVQCRAVVERRVPF IMCCGGKSSEDATDDISSGNIPVLQ KDKDNTNVNADVQKLQQQLQDIKEQ TMCPVCLDRLKNMIFLCGHG
  TCQLCGDRMSECPICRKAIERRILL YTRTRPLEQKLISEEDLAANDILDY KDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome

Gene Information

• Gene Name: MIB1 MIB E3 ubiquitin protein ligase 1 [ Homo sapiens (human) ]
• Official Symbol: MIB1
• Synonyms: MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2
• Gene ID: 57534
• mRNA Refseq: NM_020774.4
• Protein Refseq: NP_065825.1
• MIM: 608677
• UniProt ID: Q86YT6