| Species : |
Human |
| Source : |
E.coli |
| Tag : |
GST&His |
| Protein Length : |
24-307 aa |
| Form : |
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
| Bio-activity : |
MHC Class I Polypeptide Related Sequence A (MICA) is a stress-induced cell surface protein. It belongs to the MHC class I-like molecules but has distinct features. MICA is highly expressed on cells under stress, such as those with DNA damage or infected cells. Structurally, it has a domain structure that allows for interactions with other molecules.MICA can function as a ligand and binds to Killer Cell Lectin Like Receptor Subfamily D, Member 1 (KLRD1, also known as NKG2D) on the surface of natural killer (NK) cells and some T cells. This binding activates immune cells and enables them to participate in the body's immune surveillance and defense responses.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human MICA and recombinant human KLRD1. Briefly, MICA was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to KLRD1-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-MICA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human MICA and recombinant human KLRD1 was shown, the EC50 for this effect is 0.582ug/mL. |
| Molecular Mass : |
62.7kDa |
| Endotoxin : |
<1.0EU per 1μg (determined by the LAL method). |
| Purity : |
> 90% |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
| Reconstitution : |
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
