Recombinant Human MICAL2 Protein (1-495 aa), His-tagged

• Cat.No.: MICAL2-1455H
• Product Overview: Recombinant Human MICAL2 Protein (1-495 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Cell Biology. Protein Description: Partial.

Specification

• Description: Nuclear monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin and regulates the SRF signaling. Acts by modifying nuclear actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization. Acts as a key regulator of the SRF signaling pathway elicited by nerve growth factor and serum: mediates oxidation and subsequent depolymerization of nuclear actin, leading to increase MKL1/MRTF-A presence in the nucleus and promote SRF:MKL1/MRTF-A-dependent gene transcription. Does not activate SRF:MKL1/MRTF-A through RhoA.
• Source: Yeast
• Species: Human
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 58.5 kDa
• Protein length: 1-495 aa
• AA Sequence: MGENEDEKQAQAGQVFENFVQASTC KGTLQAFNILTRHLDLDPLDHRNFY SKLKSKVTTWKAKALWYKLDKRGSH KEYKRGKSCTNTKCLIVGGGPCGLR TAIELAYLGAKVVVVEKRDSFSRNN VLHLWPFTIHDLRGLGAKKFYGKFC AGSIDHISIRQLQLILFKVALMLGV EIHVNVEFVKVLEPPEDQENQKIGW RAEFLPTDHSLSEFEFDVIIGADGR RNTLEGFRRKEFRGKLAIAITANFI NRNSTAEAKVEEISGVAFIFNQKFF QDLKEETGIDLENIVYYKDCTHYFV MTAKKQSLLDKGVIINDYIDTEMLL CAENVNQDNLLSYAREAADFATNYQ LPSLDFAMNHYGQPDVAMFDFTCMY ASENAALVRERQAHQLLVALVGDSL LEPFWPMGTGCARGFLAAFDTAWMV KSWNQGTPPLELLAERESLYRLLPQ TTPENINKNFEQYTLDPGTRYPNLN SHCVRPHQVKHLYITKELEH
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: MICAL2 microtubule associated monoxygenase, calponin and LIM domain containing 2 [ Homo sapiens ]
• Official Symbol: MICAL2
• Synonyms: MICAL2; KIAA0750; MICAL-2; MICAL2PV1; MICAL2PV2; DKFZp686H2469; DKFZp686H03148
• Gene ID: 9645
• mRNA Refseq: NM_014632
• Protein Refseq: NP_055447
• MIM: 608881
• UniProt ID: O94851