Recombinant Human Mitogen-activated Protein Kinase 10, GST-His

• Cat.No.: MAPK10-691H
• Product Overview: Recombinant human JNK3 is fused at the N-terminus to a GST-His 6-thrombin cleavage site tag and expressed inS. frugiperda insect cellsusing a baculovirus expression system.

Specification

• Cat. No.: MAPK10-691H
• Description: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis.
• Source: S. frugiperda insect cells.
• Form: Liquid.
• Formulation: In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol, pH 8.0.
• Molar Mass: 82161 Da.
• Specific Activity: ≥0.1 U/mg protein.
• Unit Definition: One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Rb-CTF per min at 30 °C using variable concentrations of ATP (0.1-0.312 μM).
• Purity: ≥90% by SDS-PAGE.
• Storage: ≤ -70oC. Ship: Dry Ice Only.
• Tag: His&GST

Gene Information

• Gene Name: MAPK10 mitogen-activated protein kinase 10 [ Homo sapiens ]
• Synonyms:
  MAPK10; mitogen-activated protein kinase 10; JNK3; JNK3A; PRKM10; p493F12; FLJ12099; FLJ33785; MGC50974; p54bSAPK; MAP kinase; OTTHUMP00000161180; OTTHUMP00000161183; JNK3 alpha protein kinase; c-Jun N-terminal kinase 3; stress activated protein kinase beta; EC 2.7.11.24; MAP kinase p49 3F12; OTTHUMP00000161182; c-Jun kinase 3
• Gene ID: 5602
• mRNA Refseq: NM_002753
• Protein Refseq: NP_002744
• MIM: 602897
• UniProt ID: P53779
• Chromosome Location: 4q22.1-q23
• Pathway: Adipocytokine signaling pathway; Colorectal cancer; ErbB signaling pathway; Fc epsilon RI signaling pathway; Focal adhesion; GnRH signaling pathway; Insulin signaling pathway; MAPK signaling pathway; NOD-like receptor signaling pathway; Neurotrophin signaling pathway; Pancreatic cancer; Pathways in cancer; Progesterone-mediated oocyte maturation; RIG-I-like receptor signaling pathway; Toll-like receptor signaling pathway; Type II diabetes mellitus; Wnt signaling pathway
• Function: ATP binding; JUN kinase activity; MAP kinase activity; kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity