Recombinant Human MMAB, His-tagged

• Cat.No.: MMAB-787H
• Product Overview: Recombinant human MMAB protein (33-250aa), fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 33-250 a.a.
• Description: MMAB is a protein that catalyzes the final step in the conversion of vitamin B(12) into adenosylcobalamin (AdoCbl), a vitamin B12 containing coenzyme for methylmalonyl-CoA mutase(MCM). Impaired MMAB activity leads to the inherited disorder vitamin B12 dependent methylmalonic aciduria linked to the cblB complementation group.
• Sequences: MGSSHHHHHH SSGLVPRGSH MQSRGPQGVE DGDRPQPSSK TPRIPKIYTK TGDKGFSSTF TGERRPKDDQ VFEAVGTTDE LSSAIGFALE LVTEKGHTFA EELQKIQCTL QDVGSALATP CSSAREAHLK YTTFKAGPIL ELEQWIDKYT SQLPPLTAFI LPSGGKISSA LHFCRAVCRR AERRVVPLVQ MGETDANVAK FLNRLSDYLF TLARYAAMKE GNQEKIYKKN DPSAESEGL.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M Nacl, 1mM DTT, 10% glycerol.
• Molecular Weight: 26.3 kDa(239aa),confirmed by MALDI-TOF.
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: MMAB methylmalonic aciduria (cobalamin deficiency) cblB type [ Homo sapiens ]
• Synonyms:
  MMAB;methylmalonic aciduria (cobalamin deficiency) cblB type; ATP:cob(I)alamin adenosyltransferase; ATP:corrinoid adenosyltransferase; aquocob(I)alamin vitamin B12s adenosyltransferase; cob(I)alamin adenosyltransferase; cob(I)yrinic acid a,c-diamide adenosyltransferase, mitochondrial; methylmalonic aciduria type B protein; ATR; cob; cblB; MGC20496; EC 2.5.1.17
• Gene ID: 326625
• mRNA Refseq: NM_052845
• Protein Refseq: NP_443077
• MIM: 607568
• UniProt ID: Q96EY8
• Chromosome Location: 12q24
• Pathway: Metabolic pathways; Porphyrin and chlorophyll metabolism
• Function: ATP binding;cob(I)yrinic acid a,c-diamide adenosyltransferase activity;nucleotide binding;transferase activity