|Product Overview :||Recombinant Human MMP15(Glu47-Pro565) fused with His tag at C-terminal was expressed in NS0.|
|Description :||Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. They play critical roles in tissue remodeling, angiogenesis, tumor invasion, and rheumatoid arthritis. MMP-15, also known as MT2-MMP, is a membrane-type MMP that is expressed in many tumor tissues including urothelial carcinoma, oral cancer, ovarian carcinoma, melanoma, and astrocytoma. Structurally, MMP-15 consists of the following domains:a pro domain containing a furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplasmic tail. Recombinant Human (rh) MMP-15, consists of the pro domain, catalytic domain, hinge region and hemopexin-like domain. The pro domain contains the mutations R128P and R129G, which prevent activation by furin cleavage. Activation of rhMMP-15 is possible by treatment with rhTrypsin 3 as described in the Activity Assay Protocol.|
|Predicted N Terminal :||Glu47|
|Form :||Supplied as a 0.2 μm filtered solution in MES, NaCl and Glycerol.|
|Bio-activity :||Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog # ES010). The specific activity is >200 pmol/min/μg, as measured under the described conditions.|
|Molecular Mass :||Predicted Molecular Mass: 61 kDa;
SDS-PAGE: 60-65 kDa, reducing conditions.
|Protein length :||Glu47-Pro565|
|Endotoxin :||<1.0 EU per 1 μg of the protein by the LAL method.|
|Purity :||>70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane|
|Storage :||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.
|Shipping :||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Gene Name :||MMP15 matrix metallopeptidase 15 (membrane-inserted) [ Homo sapiens ]|
|Official Symbol :||MMP15|
|Synonyms :||MMP15; matrix metallopeptidase 15 (membrane-inserted); matrix metalloproteinase 15 (membrane inserted); matrix metalloproteinase-15; MT2 MMP; MTMMP2; SMCP 2; MMP-15; MT2MMP; MT-MMP 2; membrane-type matrix metalloproteinase 2; membrane-type-2 matrix metalloproteinase; matrix metalloproteinase 15 (membrane-inserted); SMCP-2; MT2-MMP;|
|Gene ID :||4324|
|mRNA Refseq :||NM_002428|
|Protein Refseq :||NP_002419|
|UniProt ID :||P51511|
|Chromosome Location :||16q13|
|Pathway :||Activation of Matrix Metalloproteinases, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Matrix Metalloproteinases, organism-specific biosystem;|
|Function :||calcium ion binding; enzyme activator activity; metalloendopeptidase activity; peptidase activity; protein binding; zinc ion binding;|