| Description : |
This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
73.88 kDa |
| AA Sequence : |
MEALMARGALTGPLRALCLLGCLLS HAAAAPSPIIKFPGDVAPKTDKELA VQYLNTFYGCPKESCNLFVLKDTLK KMQKFFGLPQTGDLDQNTIETMRKP RCGNPDVANYNFFPRKPKWDKNQIT YRIIGYTPDLDPETVDDAFARAFQV WSDVTPLRFSRIHDGEADIMINFGR WEHGDGYPFDGKDGLLAHAFAPGTG VGGDSHFDDDELWTLGEGQVVRVKY GNADGEYCKFPFLFNGKEYNSCTDT GRSDGFLWCSTTYNFEKDGKYGFCP HEALFTMGGNAEGQPCKFPFRFQGT SYDSCTTEGRTDGYRWCGTTEDYDR DKKYGFCPETAMSTVGGNSEGAPCV FPFTFLGNKYESCTSAGRSDGKMWC ATTANYDDDRKWGFCPDQGYSLFLV AAHEFGHAMGLEHSQDPGALMAPIY TYTKNFRLSQDDIKGIQELYGASPD IDLGTGPTPTLGPVTPEICKQDIVF DGIAQIRGEIFFFKDRFIWRTVTPR DKPMGPLLVATFWPELPEKIDAVYE APQEEKAVFFAGNEYWIYSASTLER GYPKPLTSLGLPPDVQRVDAAFNWS KNKKTYIFAGDKFWRYNEVKKKMDP GFPKLIADAWNAIPDNLDAVVDLQG GGHSYFFKGAYYLKLENQSLKSVKF GSIKSDWLGCTRTRPLEQKLISEED LAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
