Recombinant Human MMP3
Cat.No. : | MMP3-39H |
Product Overview : | Recombinant Human Matrix Metalloproteinase-3 Human Recombinant produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 230 amino acids and having a molecular mass of 22 kDa. The MMP-3 is purified by proprietary chromatographic techniques. |
- Specification
- Gene Information
- Related Products
Cat. No. : | MMP3-39H |
Description : | MMP-3 enzyme is also known as Stromelysin-1or as Transin-1 which hydrolyzes natural collagen at physiological pH and temperature. It dissolves the intervertebral nucleus pulposus and annulus fibrosus of Herniated Lumbar Intervertebral Disk . MMP-3 hydrolyzes components of the extracellular matrix like proteoglycan, laminin, fibronectin, gelatin and collagen types III, IV and IX. It also activates pro-MMP-9 and pro-MMP-8 and superactivates plasmin activated MMP-1. MMP-3 is secreted as a latent proenzyme and is activated by a variety of proteinases, e.g. plasmin, trypsin, chymotrypsin, cathepsin G or human neutrophil elastase. MMP-3 was found to be capable of activating the precursor of IL1-beta. |
Source : | Escherichia Coli. |
Physical Appearance : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity : | Greater than 98% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : | The protein was lyophilized from a concentrated (1 mg/ml) solution with no additives. |
Biological Activity : | The specific activity was found to be ≥1,250 mU/mg protein (H. Nagase, et al; J. Biol. Chem. 269, 20952, 1994). One unit is defined as the amount of enzyme that hydrolyzes 1µmol of MCA-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(DNP)-NH2 per min. at 37°C, pH 7.0. |
Solubility : | It is recommended to reconstitute the lyophilized Stromelysin-1 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
Stability : | Lyophilized Matrix Metalloproteinase-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MMP-3 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles. |
Gene Name : | MMP3 matrix metallopeptidase 3 (stromelysin 1, progelatinase) [ Homo sapiens ] |
Synonyms : | matrix metallopeptidase 3 (stromelysin 1, progelatinase); SL-1; STMY; STR1; CHDS6; MMP-3; STMY1; MGC126102; transin-1; MMP3; MGC126103; matrix metalloproteinase 3; CHDS6; Transin-1; proteoglycanase; MGC126104; Matrix metalloproteinase-3; EC 3.4.24.17 |
Gene ID : | 4314 |
mRNA Refseq : | NM_002422 |
Protein Refseq : | NP_002413 |
MIM : | 185250 |
UniProt ID : | P08254 |
Chromosome Location : | 11q22.3 |
Function : | calcium ion binding; metalloendopeptidase activity; peptidase activity; zinc ion binding |
Products Types
◆ Recombinant Protein | ||
MMP3-5433H | Recombinant Human MMP3 Protein, GST-tagged | +Inquiry |
MMP3-555H | Recombinant Human MMP3 Protein, MYC/DDK-tagged | +Inquiry |
MMP3-0029H | Recombinant Human MMP3 Protein | +Inquiry |
MMP3-1124R | Recombinant Rabbit MMP3 Protein, His-tagged | +Inquiry |
MMP3-1123H | Recombinant Human MMP3 Protein, His-tagged | +Inquiry |
◆ Native Protein | ||
MMP3-26C | Collagenase Type 3 Protein | +Inquiry |
◆ Lysates | ||
MMP3-4273HCL | Recombinant Human MMP3 293 Cell Lysate | +Inquiry |
◆ Assay kits | ||
Kit-0589 | MMP-3 Activity Assay Kit | +Inquiry |
Kit-2213 | MMP-3 Activity Fluorometric Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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