Recombinant Human MMP7 protein, His-tagged

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Recombinant Human MMP7 protein, His-tagged

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Cat.No. : MMP7-49H
Product Overview : Recombinant Human MMP7 protein (Leu18-Lys267), fused to His-tag at N-terminus, was expressed in 293F cells.
Description : This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal hemopexin domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes on chromosome 11. This gene exhibits elevated expression levels in multiple human cancers.
Source : 293F
Species : Human
Tag : His
Form : Freeze-dried powder. Buffer composition: Phosphate buffer (pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300).
Molecular Mass : Predicted Molecular Mass: 29.5 kDa. Accurate Molecular Mass: 30/18kDa as determined by SDS-PAGE reducing conditions.
Protein length : 250
Endotoxin : <1.0 EU/µg (LAL method)
Purity : > 90%
Applications : Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user).
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Concentration : Original: 200μg/mL
Reconstitution : Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
SDS-PAGE of MMP7-49H :

Gene Name : MMP7
Official Symbol : MMP7
Synonyms : MMP-7; MPSL1; PUMP-1; MAT; Matrilysin Uterine; Matrilysin; Matrin; Pump-1 Protease; Uterine Metalloproteinase
Gene ID : 4316
mRNA Refseq : NM_002423.5
Protein Refseq : NP_002414.1
MIM : 178990
UniProt ID : P09237

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