Recombinant Mononucleosomes, Symmetrically Methylated 199x601 DNA, Biotinylated
Cat.No. : | NUC-057 |
Product Overview : | Mononucleosomes assembled from recombinant histones expressed in E. coli. |
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Description : | Mononucleosomes assembled from recombinant histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P06897; H2B-P02281; H3-Q92133; H4-P62799) wrapped by 199 base pairs of DNA containing the 601 positioning sequence DNA. The the 199 bp DNA sequence contains a 147 base-pair 601 nucleosome positioning sequence, identified by Lowary and Widom, which has high affinity for histone octamers and is useful for nucleosome assembly. The 601 sequence is flanked by a 26 bp sequence as shown in application notes and contains a 5' biotin-TEG group. |
Source : | E. coli |
Tag : | N/A |
Form : | Mononucleosomes, Recombinant, Symmetrically Methylated 199x601 DNA (50 µg DNA + protein, 24.3 µg protein weight) in 10 mM Tris pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM EDTA, 20% glycerol. |
Molecular Mass : | 232,993.9 Da |
Applications : | DNA sequence with methylation sites in RED 5'-Biotin-TEGGGACCCTATACGCGGCCGCCGAATTCCTGGAGAATCCCGGTCTGCAGGCCGCTCAATTGGTCGTAGACAGCTCTACGTGGCGAATTTGCGTGCATGCGCCTGTCCCCCGCGTTTTAACCGCCA AGGGGATTACTCCCTAGTCTCCAGGCACGTGTCAGATATATACATCCTGTGGATCCGCCGGTCGCGAACAGCGACC-3' 3'CCTGGGATATGCGCCGGCGGCTTAAGGACCTCTTAGGGCCAGACGTCCGGCGAGTTAACCAGCATCTGTCGAGATGCACCGCTTAAACGCACGTACGCGGACAGGGGGCGCAAAATTGGCGGTTCCCCTAATGAGGGATCAGAGGTCCGTGCACAGTCTATATATGTAGGACACCTAGGCGGCCAGCGCTTGTCGCTGG-5' |
Storage : | Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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