Nucleosome K-AcylStat Panel
Cat.No. : | NUC-067 |
Product Overview : | A 96-well plate containing 23 unique mononucleosomes assembled from recombinant human histones expressed in E. coli. |
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Description : | A 96-well plate containing 23 unique mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908, H2B-O60814, H3.1-P68431/H3.2-Q71DI3, H4-P62805). Single and combinatorial histone post-translational modifications (PTMs) are created using a proprietary synthetic method. The nucleosomes are wrapped by 147 base pairs DNA containing a central 601-positioning sequence (Lowary and Widom 1998 J Mol Biol. (1):19-42) with a 5’ biotin-TEG group. |
Source : | E. coli |
Species : | Human |
Tag : | N/A |
Form : | The K-AcylStat panel includes an unmodified nucleosome control and 22 designer nucleosomes (dNucs: single and combinatorial PTMs), either acetylated (ac), butyrylated (bu), or crotonylated (cr) at the indicated lysine sites. |
Applications : | The access to epigenetic diversity in a physiological nucleosome context enables broad end-user applications, including nucleosome binding studies (e.g. chromatin reader binding preferences, see application data below for example using BRD4 BD-1), enzyme screening assays (e.g. identification of preferred substrates), and antibody specificity testing (e.g. for applications that require antibody specificity in a nucleosome, rather than histone peptide context). The biotin group on the DNA facilitates pull-down experiments. Mononucleosomes do not contain free DNA which could alter assayed activities. |
Storage : | Stable for six months at -80°C from date of receipt. |
Products Types
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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