Recombinant Human MRPL12 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human MRPL12 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : MRPL12-4876H
Product Overview : MRPL12 MS Standard C13 and N15-labeled recombinant protein (NP_002940) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 21.3 kDa
AA Sequence : MLPAAARPLW GPCLGLRAAA FRLARRQVPC VCAVRHMRSS GHQRCEALAG APLDNAPKEY PPKIQQLVQD IASLTLLEIS DLNELLKKTL KIQDVGLVPM GGVMSGAVPA AAAQEAVEED IPIAKERTHF TVRLTEAKPV DKVKLIKEIK NYIQGINLVQ AKKLVESLPQ EIKANVAKAE AEKIKAALEA VGGTVVLETR TRPLEQKLIS EEDLAANDIL DYKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : MRPL12 mitochondrial ribosomal protein L12 [ Homo sapiens (human) ]
Official Symbol : MRPL12
Synonyms : MRPL12; mitochondrial ribosomal protein L12; RPML12; 39S ribosomal protein L12, mitochondrial; MRPL7; MRPL7/L12; MRP-L12; 5c5-2; L12mt; MRP-L31/34; MGC8610; FLJ60124;
Gene ID : 6182
mRNA Refseq : NM_002949
Protein Refseq : NP_002940
MIM : 602375
UniProt ID : P52815

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