Recombinant Human MRPL14 Protein, GST-tagged

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Recombinant Human MRPL14 Protein, GST-tagged

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Cat.No. : MRPL14-5558H
Product Overview : Human MRPL14 full-length ORF ( NP_115487.2, 1 a.a. - 145 a.a.) recombinant protein with GST-tag at N-terminal.
Description : Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found at 17p13.3. [provided by RefSeq
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 42.3 kDa
AA Sequence : MAFFTGLWGP FTCVSRVLSH HCFSTTGSLS AIQKMTRVRV VDNSALGNSP YHRAPRCIHV YKKNGVGKVG DQILLAIKGQ KKKALIVGHC MPGPRMTPRF DSNNVVLIED NGNPVGTRIK TPIPTSLRKR EGEYSKVLAI AQNFV
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Quality Control Testing: :

12.5% SDS-PAGE Stained with Coomassie Blue.

Gene Name : MRPL14 mitochondrial ribosomal protein L14 [ Homo sapiens ]
Official Symbol : MRPL14
Synonyms : L14mt; L32mt; MRPL32; RMPL32; RPML32; MRP-L14; MRP-L32
Gene ID : 64928
mRNA Refseq : NM_032111
Protein Refseq : NP_115487
MIM : 611827
UniProt ID : Q6P1L8

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