Recombinant Human MRPL32 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human MRPL32 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : MRPL32-2259H
Product Overview : MRPL32 MS Standard C13 and N15-labeled recombinant protein (NP_114109) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 21.4 kDa
AA Sequence : MALAMLVLVV SPWSAARGVL RNYWERLLRK LPQSRPGFPS PPWGPALAVQ GPAMFTEPAN DTSGSKENSS LLDSIFWMAA PKNRRTIEVN RCRRRNPQKL IKVKNNIDVC PECGHLKQKH VLCAYCYEKV CKETAEIRRQ IGKQEGGPFK APTIETVVLY TGETPSEQDQ GKRIIERDRK RPSWFTQNTR TRPLEQKLIS EEDLAANDIL DYKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : MRPL32 mitochondrial ribosomal protein L32 [ Homo sapiens (human) ]
Official Symbol : MRPL32
Synonyms : MRPL32; mitochondrial ribosomal protein L32; L32mt; HSPC283; MRP-L32; bMRP-59b; 39S ribosomal protein L32, mitochondrial; mitochondrial large ribosomal subunit protein bL32m; EC 3.6.5.3
Gene ID : 64983
mRNA Refseq : NM_031903
Protein Refseq : NP_114109
MIM : 611839
UniProt ID : Q9BYC8

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