Active Recombinant Human MSLN protein, Fc-tagged

• Cat.No.: MSLN-340H
• Product Overview: Active Recombinant Human MSLN protein(AAH09272)(Glu 296 - Gly 580),fused with a human IgG1 Fc tag at the N-terminus, is expressed from HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 296-580 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human Mesothelin (296-580), Fc Tag at 0.5 μg/mL (100 μL/well) can bind Anti-Human MSLN Mab with a linear range of 0.8-3 ng/mL.
• Molecular Mass: The protein has a calculated MW of 59.1 kDa. The protein migrates as 60-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 0.1 EU per μg by the LAL method / rFC method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MSLN mesothelin [ Homo sapiens ]
• Official Symbol: MSLN
• Synonyms: MSLN; mesothelin; CAK1; MPF; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor; SMRP
• Gene ID: 10232
• mRNA Refseq: NM_001177355
• Protein Refseq: NP_001170826
• MIM: 601051
• UniProt ID: Q13421