Recombinant Human MTOR Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: MTOR-4973H
• Product Overview: MTOR MS Standard C13 and N15-labeled recombinant protein (NP_004949) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 288.7 kDa
• AA Sequence: MLGTGPAAATTAATTSSNVSVLQQF ASGLKSRNEETRAKAAKELQHYVTM ELREMSQEESTRFYDQLNHHIFELV SSSDANERKGGILAIASLIGVEGGN ATRIGRFANYLRNLLPSNDPVVMEM ASKAIGRLAMAGDTFTAEYVEFEVK RALEWLGADRNEGRRHAAVLVLREL AISVPTFFFQQVQPFFDNIFVAVWD PKQAIREGAVAALRACLILTTQREP KEMQKPQWYRHTFEEAEKGFDETLA KEKGMNRDDRIHGALLILNELVRIS SMEGERLREEMEEITQQQLVHDKYC KDLMGFGTKPRHITPFTSFQAVQPQ QSNALVGLLGYSSHQGLMGFGTSPS PAKSTLVESRCCRDLMEEKFDQVCQ WVLKCRNSKNSLIQMTILNLLPRLA AFRPSAFTDTQYLQDTMNHVLSCVK KEKERTAAFQALGLLSVAVRSEFKV YLPRVLDIIRAALPPKDFAHKRQKA MQVDATVFTCISMLARAMGPGIQQD IKELLEPMLAVGLSPALTAVLYDLS RQIPQLKKDIQDGLLKMLSLVLMHK PLRHPGMPKGLAHQLASPGLTTLPE ASDVGSITLALRTLGSFEFEGHSLT QFVRHCADHFLNSEHKEIRMEAART CSRLLTPSIHLISGHAHVVSQTAVQ VVADVLSKLLVVGITDPDPDIRYCV LASLDERFDAHLAQAENLQALFVAL NDQVFEIRELAICTVGRLSSMNPAF VMPFLRKMLIQILTELEHSGIGRIK EQSARMLGHLVSNAPRLIRPYMEPI LKALILKLKDPDPDPNPGVINNVLA TIGELAQVSGLEMRKWVDELFIIIM DMLQDSSLLAKRQVALWTLGQLVAS TGYVVEPYRKYPTLLEVLLNFLKTE QNQGTRREAIRVLGLLGALDPYKHK VNIGMIDQSRDASAVSLSESKSSQD SSDYSTSEMLVNMGNLPLDEFYPAV SMVALMRIFRDQSLSHHHTMVVQAI TFIFKSLGLKCVQFLPQVMPTFLNV IRVCDGAIREFLFQQLGMLVSFVKS HIRPYMDEIVTLMREFWVMNTSIQS TIILLIEQIVVALGGEFKLYLPQLI PHMLRVFMHDNSPGRIVSIKLLAAI QLFGANLDDYLHLLLPPIVKLFDAP EAPLPSRKAALETVDRLTESLDFTD YASRIIHPIVRTLDQSPELRSTAMD TLSSLVFQLGKKYQIFIPMVNKVLV RHRINHQRYDVLICRIVKGYTLADE EEDPLIYQHRMLRSGQGDALASGPV ETGPMKKLHVSTINLQKAWGAARRV SKDDWLEWLRRLSLELLKDSSSPSL RSCWALAQAYNPMARDLFNAAFVSC WSELNEDQQDELIRSIELALTSQDI AEVTQTLLNLAEFMEHSDKGPLPLR DDNGIVLLGERAAKCRAYAKALHYK ELEFQKGPTPAILESLISINNKLQQ PEAAAGVLEYAMKHFGELEIQATWY EKLHEWEDALVAYDKKMDTNKDDPE LMLGRMRCLEALGEWGQLHQQCCEK WTLVNDETQAKMARMAAAAAWGLGQ WDSMEEYTCMIPRDTHDGAFYRAVL ALHQDLFSLAQQCIDKARDLLDAEL TAMAGESYSRAYGAMVSCHMLSELE EVIQYKLVPERREIIRQIWWERLQG CQRIVEDWQKILMVRSLVVSPHEDM RTWLKYASLCGKSGRLALAHKTLVL LLGVDPSRQLDHPLPTVHPQVTYAY MKNMWKSARKIDAFQHMQHFVQTMQ QQAQHAIATEDQQHKQELHKLMARC FLKLGEWQLNLQGINESTIPKVLQY YSAATEHDRSWYKAWHAWAVMNFEA VLHYKHQNQARDEKKKLRHASGANI TNATTAATTAATATTTASTEGSNSE SEAESTENSPTPSPLQKKVTEDLSK TLLMYTVPAVQGFFRSISLSRGNNL QDTLRVLTLWFDYGHWPDVNEALVE GVKAIQIDTWLQVIPQLIARIDTPR PLVGRLIHQLLTDIGRYHPQALIYP LTVASKSTTTARHNAANKILKNMCE HSNTLVQQAMMVSEELIRVAILWHE MWHEGLEEASRLYFGERNVKGMFEV LEPLHAMMERGPQTLKETSFNQAYG RDLMEAQEWCRKYMKSGNVKDLTQA WDLYYHVFRRISKQLPQLTSLELQY VSPKLLMCRDLELAVPGTYDPNQPI IRIQSIAPSLQVITSKQRPRKLTLM GSNGHEFVFLLKGHEDLRQDERVMQ LFGLVNTLLANDPTSLRKNLSIQRY AVIPLSTNSGLIGWVPHCDTLHALI RDYREKKKILLNIEHRIMLRMAPDY DHLTLMQKVEVFEHAVNNTAGDDLA KLLWLKSPSSEVWFDRRTNYTRSLA VMSMVGYILGLGDRHPSNLMLDRLS GKILHIDFGDCFEVAMTREKFPEKI PFRLTRMLTNAMEVTGLDGNYRITC HTVMEVLREHKDSVMAVLEAFVYDP LLNWRLMDTNTKGNKRSRTRTDSYS AGQSVEILDGVELGEPAHKKTGTTV PESIHSFIGDGLVKPEALNKKAIQI INRVRDKLTGRDFSHDDTLDVPTQV ELLIKQATSHENLCQCYIGWCPFWT RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: MTOR mechanistic target of rapamycin kinase [ Homo sapiens (human) ]
• Official Symbol: MTOR
• Synonyms: MTOR; mechanistic target of rapamycin (serine/threonine kinase); FK506 binding protein 12 rapamycin associated protein 1, FRAP, FRAP1, FRAP2; serine/threonine-protein kinase mTOR; dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1); FK506 binding protein 12 rapamycin associated protein 2; FKBP rapamycin associated protein; FKBP12 rapamycin complex associated protein 1; FLJ44809; mammalian target of rapamycin; RAFT1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; rapamycin target protein; RAPT1; rapamycin target protein 1; FKBP-rapamycin associated protein; FKBP12-rapamycin complex-associated protein 1; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FRAP; FRAP1; FRAP2
• Gene ID: 2475
• mRNA Refseq: NM_004958
• Protein Refseq: NP_004949
• MIM: 601231
• UniProt ID: P42345