Recombinant Human MTOR protein, His & T7-tagged

• Cat.No.: MTOR-1820H
• Product Overview: Recombinant Human MTOR aa. (Ala2226~Val2488 (Accession # P42345)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.7kDa.
• Protein length: Ala2226~Val2488 (Accession # P42345)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: MTOR mechanistic target of rapamycin [ Homo sapiens (human) ]
• Official Symbol: MTOR
• Synonyms: AGL; amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase; GDE; glycogen debranching enzyme; amylo-1, 6-glucosidase, 4-alpha-glucanotransferase; amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferaseprovided; glycogen debrancher; glycogen debranching protein
• Gene ID: 2475
• mRNA Refseq: NM_004958.3
• Protein Refseq: NP_004949.1
• UniProt ID: P42345