Recombinant Human MUC2 Protein (36-240 aa), His-tagged

• Cat.No.: MUC2-1466H
• Product Overview: Recombinant Human MUC2 Protein (36-240 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Signal Transduction. Protein Description: Partial.

Specification

• Description: Coats the epithelia of the intestines, airways, and other mucus mbrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
• Source: Yeast
• Species: Human
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 24.8 kDa
• Protein length: 36-240 aa
• AA Sequence: VCSTWGNFHYKTFDGDVFRFPGLCD YNFASDCRGSYKEFAVHLKRGPGQA EAPAGVESILLTIKDDTIYLTRHLA VLNGAVVSTPHYSPGLLIEKSDAYT KVYSRAGLTLMWNREDALMLELDTK FRNHTCGLCGDYNGLQSYSEFLSDG VLFSPLEFGNMQKINQPDVVCEDPE EEVAPASCSEHRAECERLLTAEAFA DCQDL
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: MUC2 mucin 2, oligomeric mucus/gel-forming [ Homo sapiens ]
• Official Symbol: MUC2
• Synonyms: MUC2; mucin-2; MLP; SMUC; MUC-2
• Gene ID: 4583
• mRNA Refseq: NM_002457
• Protein Refseq: NP_002448
• MIM: 158370
• UniProt ID: Q02817

Reviews

11/10/2020

It was observed that the expression level was high enough to meet the experimental requirements.

05/08/2020

The use of this protein found high purity and no significant impurities.

01/31/2020

Relatively active and able to efficiently perform the required biological activity experiments.

Q&As

What is the transcriptional regulation mechanism of MUC2 protein?

The transcriptional regulation of MUC2 protein is regulated by a variety of signaling pathways and transcription factors, including methylation modification in the MUC2 promoter region, and regulation of transcription factors GATA4 and KLF4.

Are mutations in the MUC2 protein associated with hereditary myxous bowel disease?

Yes, mutations in the MUC2 protein have been found to be associated with hereditary myxous enteropathy (HM), a rare intestinal disease caused primarily by mutations in the MUC2 protein that result in loss of function of the mucus layer.

What is the role of the MUC2 protein in intestinal immunoregulation?

MUC2 protein is involved in intestinal immune regulation by influencing intestinal microbes, regulating inflammatory response and antigen presentation, helping to maintain intestinal immune balance.

How does the shear variant of the MUC2 protein affect its function?

The shear variant of the MUC2 protein may have an important impact on its mucus layer formation and stability. Different shear variants may affect their adhesion and resistance to degradation.

What is the relationship between MUC2 protein and intestinal inflammation?

Abnormal expression and functional defects of the MUC2 protein may lead to increased retention of microorganisms and harmful substances in the intestine, triggering intestinal inflammatory responses such as intestinal mucosal inflammation and destruction of the mucus layer.

How does MUC2 protein function in the intestinal mucosal barrier?

By forming a mucus layer, MUC2 protein provides a protective barrier to the gut against harmful microorganisms, toxins and other external irritants from entering the intestinal mucosa.