| Product overview : |
Recombinant Human MYC (P01106) aa. (Ser184~Ala454) fused with His & GST tag was produced in E. coli cells. |
| Description : |
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
His & GST |
| Form : |
Freeze-dried powder |
| Molecular Mass : |
Predicted Molecular Mass: 60.2kDa; Accurate Molecular Mass: 65kDa as determined by SDS-PAGE reducing conditions. |
| Protein length : |
Ser184~Ala454 |
| Purity : |
>98% |
| Characteristic : |
Isoelectric point is 6.6. |
| Applications : |
Cell culture; Activity Assays; In vivo assays. |
| Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
| Storage buffer : |
20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300. |
| Reconstitution : |
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex |
