Recombinant Human Myosin Light Chain, Phosphorylatable, Fast Skeletal Muscle, His-tagged

• Cat.No.: MYLPF-5081H
• Product Overview: Recombinant human MYLPF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: Myosin regulatory light chains, including MRCL3, MYLPF and MYL9, regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases the actin-activated myosin ATPase activity, thereby regulating the contractile activity. MYLPF is critically important for fast and slow skeletal muscle development.
• Concentration: 0.25 mg/ml
• Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 10% glycerol and 100mM NaCl.
• Purity: > 85% by SDS - PAGE
• Sequence: 1-169 amino acids: MGSSHHHHHH SSGLVPRGSH MAPKRAKRRT VEGGSSSVFS MFDQTQIQEF KEAFTVIDQN RDGIIDKEDL RDTFAAMGRL NVKNEELDAM MKEASGPINF TVFLTMFGEK LKGADPEDVI TGAFKVLDPE GKGTIKKKFL EELLTTQCDR FSQEEIKNMW AAFPPDVGGN VDYKNICYVI THGDAKDQE
• Molecular Mass: 21.2 kDa (189aa), confirmed by MALDI-TOF
• Applications: SDS-PAGE
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: MYLPF myosin light chain, phosphorylatable, fast skeletal muscle [ Homo sapiens ]
• Official Symbol: MYLPF
• Synonyms: myosin light chain, phosphorylatable, fast skeletal muscle; MRLC2; MYL11; HUMMLC2B; MGC13450; DKFZp779C0757; MYLPF; myosin light chain 2; myosin regulatory light chain 2; fast skeletal myosin light chain 2; myosin, light chain 11, regulatory; MLC2B; Fast skeletal myosin light chain 2
• Gene ID: 29895
• mRNA Refseq: NM_013292
• Protein Refseq: NP_037424
• UniProt ID: Q96A32
• Chromosome Location: 16p11.2
• Pathway: ErbB1 downstream signaling; Focal adhesion; Leukocyte transendothelial migration; Regulation of actin cytoskeleton; Tight junction
• Function: calcium ion binding; structural constituent of muscle