Recombinant Human NAD Kinase, His-tagged

• Cat.No.: NADK-613H
• Product Overview: Recombinant Human NADK (aa 64-446)produced inE. coli is fused at the N-terminus to a His-tag.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 64-446 a.a.
• Description: NAD+ kinase (EC 2.7.1.23, NADK) is an enzyme that converts nicotinamide adenine dinucleotide (NAD+) into NADP+, through phosphorylating the NAD+ coenzyme. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. The structure of the NADK from the archaean Archaeoglobus fulgidus has been determined. In humans, NAD+ kinase is encoded by the NADK gene.
• Purity: ≥95% (SDS-PAGE).
• Purity Detail: Free of any nucleotide converting side-activities.
• Quantity: Sufficient for the preparation of >10µmol of NADP from NAD and ATP in 1ml of a 10mM solution.
• Formulation: Liquid. Ammonium sulphate suspension.
• Reconstitution: the pellet in 50mM TRIS/HCl pH 7.5, 150mM NaCl, 1mM DTT.
• Specific Activity: ≥3U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1µmol of NADP per min.
• Short Term Storage: +4°C.
• Long Term Storage: -20°C.
• Use/Stability: Stable for at least 3 months when stored at +4°C.
• Handling: Avoid freeze/thaw cycles.

Gene Information

• Gene Name: NADK NAD kinase [ Homo sapiens ]
• Synonyms: NAD kinase; FLJ13052; FLJ37724; dJ283E3.1; RP1-283E3.6; NADK; poly(P)/ATP NAD kinase; OTTHUMP00000001110; EC 2.7.1.23
• Gene ID: 65220
• mRNA Refseq: NM_023018
• Protein Refseq: NP_075394
• MIM: 611616
• UniProt ID: O95544
• Chromosome Location: 1p36.33-p36.21
• Pathway: Metabolic pathways; Nicotinate and nicotinamide metabolism; Metabolism of vitamins and cofactors
• Function: ATP binding; NAD+ kinase activity; metal ion binding; nucleotide binding; protein binding; transferase activity