Recombinant Human NECTIN3 protein, Fc & Avi-tagged, Biotinylated

• Cat.No.: NECTIN3-0633H
• Product Overview: Biotinylated Human Nectin-3, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Gly 58 - Asp 400 (Accession # Q9NQS3-1). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Description: Poliovirus receptor-related 3 (PVRL3), also known as nectin-3 and CD113, is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. Nectins are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin system in epithelial cells. PVRL3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Furthermore, PVRL3 induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation.
• Source: HEK293
• Species: Human
• Tag: Fc & Avi
• Predicted N Terminal: Leu 56 & Gly 58
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 66.1 kDa. The protein migrates as 90-95 kDa and 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Gly 58 - Asp 400
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: Powder,RT

Gene Information

• Gene Name: NECTIN3 nectin cell adhesion molecule 3 [ Homo sapiens (human) ]
• Official Symbol: NECTIN3
• Synonyms: PPR3; PRR3; CD113; PVRL3; PVRR3; CDW113; NECTIN-3; nectin 3; poliovirus receptor-related 3; poliovirus receptor-related protein 3; nectin-3
• Gene ID: 25945
• mRNA Refseq: NM_001243286.1
• Protein Refseq: NP_001230215.1
• UniProt ID: Q9NQS3