| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Fc |
| Protein Length : |
Gly 58 - Asp 400 |
| Description : |
Poliovirus receptor-related 3 (PVRL3), also known as nectin-3 and CD113, is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. Nectins are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin system in epithelial cells. PVRL3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Furthermore, PVRL3 induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. |
| Predicted N Terminal : |
Gly 58 |
| Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5, 10% trehalose. |
| Molecular Mass : |
Human Nectin-3, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 64.3 kDa. The reducing (R) protein migrates as 80-100 kDa in SDS-PAGE due to glycosylation. |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
| Shipping : |
Powder,RT |
