| Description : |
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
98.2 kDa |
| AA Sequence : |
MERPYTFKDFLLRPRSHKSRVKGFL RLKMAYMPKNGGQDEENSDQRDDME HGWEVVDSNDSASQHQEELPPPPLP PGWEEKVDNLGRTYYVNHNNRTTQW HRPSLMDVSSESDNNIRQINQEAAH RRFRSRRHISEDLEPEPSEGGDVPE PWETISEEVNIAGDSLGLALPPPPA SPGSRTSPQELSEELSRRLQITPDS NGEQFSSLIQREPSSRLRSCSVTDA VAEQGHLPPPSAPAGRARSSTVTGG EEPTPSVAYVHTTPGLPSGWEERKD AKGRTYYVNHNNRTTTWTRPIMQLA EDGASGSATNSNNHLIEPQIRRPRS LSSPTVTLSAPLEGAKDSPVRRAVK DTLSNPQSPQPSPYNSPKPQHKVTQ SFLPPGWEMRIAPNGRPFFIDHNTK TTTWEDPRLKFPVHMRSKTSLNPND LGPLPPGWEERIHLDGRTFYIDHNS KITQWEDPRLQNPAITGPAVPYSRE FKQKYDYFRKKLKKPADIPNRFEMK LHRNNIFEESYRRIMSVKRPDVLKA RLWIEFESEKGLDYGGVAREWFFLL SKEMFNPYYGLFEYSATDNYTLQIN PNSGLCNEDHLSYFTFIGRVAGLAV FHGKLLDGFFIRPFYKMMLGKQITL NDMESVDSEYYNSLKWILENDPTEL DLMFCIDEENFGQTYQVDLKPNGSE IMVTNENKREYIDLVIQWRFVNRVQ KQMNAFLEGFTELLPIDLIKIFDEN ELELLMCGLGDVDVNDWRQHSIYKN GYCPNHPVIQWFWKAVLLMDAEKRI RLLQFVTGTSRVPMNGFAELYGSNG PQLFTIEQWGSPEKLPRAHTCFNRL DLPPYETFEDLREKLLMAVENAQGF EGVDTRTRPLEQKLISEEDLAANDI LDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
