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Recombinant Human NEFM protein, His-tagged

Cat.No. : NEFM-6816H
Product Overview : Recombinant Human NEFM aa. (Leu105~Gln287) fused with N-terminal His tag was produced in E. coli cells.
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  • Gene Information
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Description : Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Source : E. coli
Species : Human
Tag : His
Form : Freeze-dried powder
Molecular Mass : 26kDa as determined by SDS-PAGE reducing conditions.
Protein length : Leu105~Gln287
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 5.5.
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200ug/mL
Storage buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
Gene Name : NEFM neurofilament medium [ Homo sapiens (human) ]
Official Symbol : NEFM
Synonyms : NFM; NEF3; NF-M; 160 kDa neurofilament protein; neurofilament 3; neurofilament triplet M protein; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); neurofilament medium polypeptide
Gene ID : 4741
mRNA Refseq : NM_001105541.1
Protein Refseq : NP_001099011.1
UniProt ID : Q9UK51

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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