Recombinant Human NELFB Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human NELFB Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : NELFB-4504H
Product Overview : COBRA1 MS Standard C13 and N15-labeled recombinant protein (NP_056271) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2), either NELFC or NELFD (TH1L), and NELFE (RDBP). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1) and SPT5 (SUPT5H), to cause transcriptional pausing of RNA polymerase II.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 65.5 kDa
AA Sequence : MFAGLQDLGV ANGEDLKETL TNCTEPLKAI EQFQTENGVL LPSLQSALPF LDLHGTPRLE FHQSVFDELR DKLLERVSAI ASEGKAEERY KKLEDLLEKS FSLVKMPSLQ PVVMCVMKHL PKVPEKKLKL VMADKELYRA CAVEVKRQIW QDNQALFGDE VSPLLKQYIL EKESALFSTE LSVLHNFFSP SPKTRRQGEV VQRLTRMVGK NVKLYDMVLQ FLRTLFLRTR NVHYCTLRAE LLMSLHDLDV GEICTVDPCH KFTWCLDACI RERFVDSKRA RELQGFLDGV KKGQEQVLGD LSMILCDPFA INTLALSTVR HLQELVGQET LPRDSPDLLL LLRLLALGQG AWDMIDSQVF KEPKMEVELI TRFLPMLMSF LVDDYTFNVD QKLPAEEKAP VSYPNTLPES FTKFLQEQRM ACEVGLYYVL HITKQRNKNA LLRLLPGLVE TFGDLAFGDI FLHLLTGNLA LLADEFALED FCSSLFDGFF LTASPRKENV HRHALRLLIH LHPRVAPSKL EALQKALEPT GQSGEAVKEL YSQLGEKLEQ LDHRKPSPAQ AAETPALELP LPSVPAPAPL TRTRPLEQKL ISEEDLAAND ILDYKDDDDK V
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : NELFB negative elongation factor complex member B [ Homo sapiens (human) ]
Official Symbol : NELFB
Synonyms : NELFB; COBRA1; NELF-B; RP13-122B23.3; negative elongation factor complex member B; negative elongation factor B; cofactor of BRCA1; negative elongation factor protein B
Gene ID : 25920
mRNA Refseq : NM_015456
Protein Refseq : NP_056271
MIM : 611180
UniProt ID : Q8WX92

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