Recombinant Human NENF protein, His-tagged

• Cat.No.: NENF-1263H
• Product Overview: Recombinant Human NENF protein(33-172 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 33-172 aa
• Form: The purified protein was lyophilized from sterile phosphate-buffered saline (PBS: 58 mM Na₂HPO₄, 17 mM NaH₂PO₄, 68 mM NaCl, pH 7.4). Prior to lyophilization, 5% (w/v) trehalose and 5% (w/v) mannitol were incorporated as cryoprotective excipients. The protein was eluted with a buffer containing 300 mM imidazole.
• AASequence: QTPRPAERGPPVRLFTEEELARYGGEEEDQPIYLAVKGVVFDVTSGKEFYGRGAPYNALTGKDSTRGVAKMSLDPADLTHDTTGLTAKELEALDEVFTKVYKAKYPIVGYTARRILNEDGSPNLDFKPEDQPHFDIKDEF
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Dissolve the product in 200 μl sterile water to achieve a working concentration of 0.25 µg/μl. If experimental compatibility allows, mix the aqueous solution with an equal volume of glycerol (1:1 ratio) after initial reconstitution.

Gene Information

• Gene Name: NENF neudesin neurotrophic factor [ Homo sapiens ]
• Official Symbol: NENF
• Synonyms: NENF; neudesin neurotrophic factor; neuron derived neurotrophic factor; neudesin; CIR2; SCIRP10; SPUF; SCIRP10-related protein; cell growth-inhibiting protein 47; neuron-derived neurotrophic factor; secreted protein of unknown function; Spinal cord injury related protein 10; cell immortalization-related protein 2
• Gene ID: 29937
• mRNA Refseq: NM_013349
• Protein Refseq: NP_037481
• MIM: 611874
• UniProt ID: Q9UMX5