Cat.No. : |
NTF3-22H
Optional Service: Optional requirements on this protein
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Product Overview : |
Recombinant Human Neurotrophin 3 encoding the NT-3 prepropeptide was inserted into a baculovirus expression vector and expressed inSf 21insect cells. |
Description : |
Neurotrophin 3 (NT-3), also known as NTF3 is a basic protein found in neurons of the central nervous system. NT-3 selectively supports the survival of neuronal cell populations. It rapidly potentiate the spontaneous and impulse-evoked synaptic activity of developing neuromuscular synapses in culture and thus appears to be involved in the regulation of functions of developing synapses. NT-3 negatively modulates nerve growth factor (NGF) receptor expression and associated nociceptive phenotype in intact neurons, suggesting a beneficial role in treating aspects of neuropathic pain mediated by NGF. NT-3 is effective at suppressing thermal hyperalgesia associated with chronic constriction injury (CCI). |
Molecular Weight : |
The predicted molecular weight of Recombinant Human NT-3 is Mr 13.6 kDa. |
Source : |
Sf 21 Insect Cells. |
State Of Matter : |
Lyophilized. |
Purity : |
>97% by SDS Page and analyzed by silver stain. |
Endotoxin : |
<1.0 EU/µg as determined by the LAL method. |
Biological Activity : |
The biological activity of Human NT-3 was determined by its ability to stimulate proliferation of the TrkB-transfected cell line, BaF-TrkB-BD. The expected ED50 for this effect is typically 10 - 30 ng/mL. |
Storage And Stability : |
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. |
PDB rendering based on 1b8k. : |
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