Recombinant Human NFATC1 Protein, Full Length, C-Flag tagged

• Cat.No.: NFATC1-20HFL
• Product Overview: Recombinant protein of human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATC1), transcript variant 1 with a C-Flag tag was expressed in HEK293T.

Specification

• Description: The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.
• Source: HEK293T
• Species: Human
• Tag: Flag
• Protein length: Full Length
• Molecular Mass: 77.6 kDa
• AA Sequence: MPSTSFPVPSKFPLGPAAAVFGRGE TLGPAPRAGGTMKSAEEEHYGYASS NVSPALPLPTAHSTLPAPCHNLQTS TPGIIPPADHPSGYGAALDGGPAGY FLSSGHTRPDGAPALESPRIEITSC LGLYHNNNQFFHDVEVEDVLPSSKR SPSTATLSLPSLEAYRDPSCLSPAS SLSSRSCNSEASSYESNYSYPYASP QTSPWQSPCVSPKTTDPEEGFPRGL GACTLLGSPRHSPSTSPRASVTEES WLGARSSRPASPCNKRKYSLNGRQP PYSPHHSPTPSPHGSPRVSVTDDSW LGNTTQYTSSAIVAAINALTTDSSL DLGDGVPVKSRKTTLEQPPSVALKV EPVGEDLGSPPPPADFAPEDYSSFQ HIRKGGFCDQYLAVPQHPYQWAKPK PLSPTSYMSPTLPALDWQLPSHSGP YELRIEVQPKSHHRAHYETEGSRGA VKASAGGHPIVQLHGYLENEPLMLQ LFIGTADDRLLRPHAFYQVHRITGK TVSTTSHEAILSNTKVLEIPLLPEN SMRAVIDCAGILKLRNSDIELRKGE TDIGRKNTRVRLVFRVHVPQPSGRT LSLQVASNPIECSQRSAQELPLVEK QSTDSYPVVGGKKMVLSGHNFLQDS KVIFVEKAPDGHHVWEMEAKTDRDL CKPNSLVVEIPPFRNQRITSPVHVS FYVCNGKRKRSQYQRFTYLPANGNA IFLTVSREHERVGCFFSGPTRTRPL EQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Notes: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: > 0.05 μg/μL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol

Gene Information

• Gene Name: NFATC1 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 [ Homo sapiens (human) ]
• Official Symbol: NFATC1
• Synonyms: NFATC1; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1; nuclear factor of activated T-cells, cytoplasmic 1; NF ATC; NFAT2; NFATc; NF-ATc1; NFAT transcription complex cytosolic component; NF-ATC; MGC138448
• Gene ID: 4772
• mRNA Refseq: NM_172390
• Protein Refseq: NP_765978
• MIM: 600489
• UniProt ID: O95644